Figure. Timelapse movies of ZO1 in tight junctions. Timelapse movies of live hiPS cells expressing mEGFP tagged tight junction protein ZO-1 imaged on a spinning-disk confocal microscope. Images were collected in 3D every 3 minutes for 1.5hours (left) or for 15 hours (right). Images are maximum intensity projections; playback speed is 910x (left) and 1800x (right) real time.
Observations and descriptions from the microscope