Request abstract from Gene-editing
Using new gene editing technology, we are able to tag and mutate genes with greater precision than ever before, creating stable cell lines endogenously expressing markers in discrete proteins associated with major cellular structures and organelles. We can then induce these cells to become any kind of cell through various differentiation steps, carrying with them through their journey these unique markers. This allows us to use live cell imaging approaches to make valuable observations about cell organization and activities as the cell divides and replicates prior to specializing toward any particular lineage, and then as it goes through terminal differentiation to a mature specialized cell type. How the cell repurposes an essentially finite set of tools into an array of specialized systems to serve the unique purposes of each cell type then begins to emerge. Using specialized 3D analysis approaches in conjunction with modeling and machine learning approaches, we can integrate the information we are generating into an interactive 3D animation based on data from studies in real cells. These lines and the analysis and modeling approaches will be available to the community as part of our open access policy.