Figures. Z-stack and Timelapse movies of Lysosomes. A) Z-stack of live hiPS cells expressing mEGFP tagged LAMP1 imaged on a spinning-disk confocal microscope. Movie starts at the bottom of the cells and ends at the top. Scale bar is 5 microns. B–C) Timelapse movie of live hiPS cells expressing mEGFP tagged LAMP1. Images were taken as a single slice near the top (B) or bottom (C) of the cell every 1 second for 100 seconds on a spinning-disk confocal microscope. Images have had the ‘despeckle’ ImageJ filter applied. Movie sped up 10x over real time. Scale bar is 5 microns.
Observations and descriptions from the microscope