Gene edited cells differentiate into cardiomyocytes

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B. 

C. 

Figure 1. After differentiation of the edited stem cells into cardiomyocytes, cells begin to beat spontaneously. Three videos taken at day 16 of differentiation show a variety of different rates and behaviors: (A) small contractile pulsing of the tissue, which remains attached to the bottom of the culture dish. (B) Wavelike, synchronous beating throughout the cardiac tissue, which has lifted off the bottom of the culture dish in most regions in this field of view. (C) Similar wavelike beating as seen in (B), however more vigorous and at a faster rate.

Playback speed is real time and each video is roughly 2.5mm across the field of view, taken from a standard camera looking through the microscope eyepiece.

Figure 2. Many structures reorganize during differentiation. α-actinin found primarily in the rings and fiber bundles at the top and bottom of hiPSCs respectively are reorganized into the contractile apparatus of sarcomeres in cardiomyocytes.