Allen Cell Methods
Instructional videos for success in the lab

To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, researchers from our teams highlight nuanced techniques and helpful tips while demonstrating various laboratory protocols.
Read our paper on Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization in Molecular Biology of the Cell (MBoC).
ALLEN CELL METHODS
1. Thawing human iPS Cells in 1 mL Greiner Bio-One Cryo.S Vials - [Vial B]
To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, our Erik Ehlers highlights nuanced techniques and helpful tips while demonstrating how to thaw a vial of hiPS cells the 1 mL Greiner Bio-One Cryo.S Vials. This vial type is referenced as vial "B" in our updated WTC culture protocol.
ALLEN CELL METHODS
2. Thawing human iPS cells - [Vial A]
To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, our Jacqueline Smith highlights nuanced techniques and helpful tips while demonstrating how to thaw a vial of hiPS cells. This vial type is referenced as vial "A" in our updated WTC culture protocol.
ALLEN CELL METHODS
3. Culturing & maintaining hiPSCs
Amanda Haupt demonstrates our single cell passaging protocol for gene editing human induced pluripotent stem cells.
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This process should take 15–30 minutes to complete and we recommend passaging a maximum of two cell lines at a time in parallel. Researchers can obtain our publicly available gene-edited fluorescently tagged cell lines through our Allen Cell Catalog.
ALLEN CELL METHODS
4. Gene editing: RNP transfection for gene-editing hiPSCs
Maggie Fuqua demonstrates our RNP transfection protocol for gene editing human induced pluripotent stem cells.

​This process should take 30–60 minutes to complete. We recommend preparing all reagents and materials beforehand. Our researchers have used the RNP transfection method for all of the gene-edited hiPS cells available through our Allen Cell Catalog.
JOVE VIDEO JOURNAL
5. Gene editing: Endogenous Protein Tagging in hiPSCs Using CRISPR/Cas9
This Jove Video Journal article presents a protocol we developed for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.

Read more about our methods & standard operating procedures. Researchers can obtain these hiPS cells or plasmids through our Cell Catalog.
​ALLEN CELL METHODS
6. Cardiomyocyte Differentiation of hiPSCs
Angel Nelson highlights nuanced techniques and helpful tips while demonstrating our cardiac differentiation protocol for the WTC parental line and our gene-edited cells.

If the experiment is successful, you can expect to see beating cardiomyocytes at day 7. Researchers can obtain the hiPS cells used in this protocol through our Allen Cell Catalog.
​ALLEN CELL METHODS
7. Techniques while working with Matrigel
Angel Nelson highlights nuanced techniques and helpful tips while working with Matrigel.

Researchers can obtain the hiPS cells used in this protocol through our Allen Cell Catalog.
​ALLEN CELL METHODS
8. Cryopreservation of clonal human iPS cell lines in 96-well plates
Amanda Haupt highlights helpful tips while demonstrating how to cryopreserve clonal human induced pluripotent stem cells in 96-well plates.

Researchers can obtain the hiPS cells used in this protocol through our Allen Cell Catalog.
​ALLEN CELL METHODS
9. Automated tissue culture platform
Mackenzie Coston showcases the automated tissue culture platform we use to seed, passage, feed, and maintain our cells.​

Researchers can obtain the hiPS cells used in this protocol through our Allen Cell Catalog.
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