Protocols published on Protocols.io

Illumination Power and illumination stability
Protocols.io, February 2022. Nathalie G, Steve B, Rodrigo RB, Fabio B, Laszlo B, Veronika B, Ulrike B, Manel B, Craig B, Mariana TC, Pina C, Richard C, Nasser DM, Sam D, Frank E, Orestis F, Andreas F, Manfred G, David G, Marcel K, Birgit H, Gabriel K, Alex JL, Jeffrey MLD, Pascal L, Miso M, Michael SN, Britta SD, Stanley S, Sathya S, Roland T, Tse-Luen W, Kees VDO, Chloë VOT, Roland N, Laurent G. 
Maintenance of Undifferentiated hiPSC Cultures and Differentiation to Cardiomyocytes on Glass Surfaces
Protocols.io, March 2021. Angelique N, Stephanie QD, Kaytlyn G, Melissa H, Caroline H, Ruwanthi NG.

Standard operating procedures for cells in the lab

SOP: Cardiomyocyte differentiation methods v1.2.pdf
File Size: 633 kb
File Type: pdf
Download File
SOP: Fluorescent dye solution for flat field correction v1.0.pdf
File Size: 273 kb
File Type: pdf
Download File
SOP: Cell line scaleup & banking v1.3.pdf
File Size: 635 kb
File Type: pdf
Download File
SOP: CellMask and NucBlue v1.0.pdf
File Size: 11249 kb
File Type: pdf
Download File
SOP: WTC culture v1.8.pdf
File Size: 736 kb
File Type: pdf
Download File
updated:
May 2024
SOP: RNP transfection v1.0.pdf
File Size: 2156 kb
File Type: pdf
Download File
SOP: Cell plating for imaging v1.0.pdf
File Size: 2750 kb
File Type: pdf
Download File
SOP: ddPCR CNV assay for screening clones v1.0.pdf
File Size: 2963 kb
File Type: pdf
Download File

Standard operating procedures for microscopy

SOP: Sub-resolution and focal check beads solution for alignment and psf measurements v1.0.pdf
File Size: 142 kb
File Type: pdf
Download File
SOP: Microscopy pipeline workflow image acquisition v1.0.pdf
File Size: 1793 kb
File Type: pdf
Download File
SOP: Fluorescent dye solution for flat field correction v1.0.pdf
File Size: 273 kb
File Type: pdf
Download File
SOP: Automated 96-well plate matrigel coating.pdf
File Size: 258 kb
File Type: pdf
Download File
SOP: Automated 96-well plate seeding.pdf
File Size: 252 kb
File Type: pdf
Download File
SOP: Automated daily feeding & imaging of ipscs.pdf
File Size: 425 kb
File Type: pdf
Download File
Allen Institute for Cell Science is a part of the Allen Institute. The mission of the Allen Institute is to understand the principles that govern life, and to advance health. Our creative and multi-dimensional teams focus on answering some of the biggest questions in bioscience. We accelerate foundational research, catalyze bold ideas, develop tools and models, and openly share our science to make a broad, transformational impact on the world.
Follow Us  
​Copyright © 2025 Allen Institute. All Rights Reserved.