ALLEN CELL EXPLORER
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      About our hiPS cells
      1. hiPS Cell Structure Overview
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      3. Cell structure observations
      4. Why endogenous tagging?
      5. Differentiation into cardiomyocytes
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      7. Download cell data: Images, genomics, & features
  • Data & Digital Tools
      General
      1. Tools and resources overview
      2. Download cell data (images, genomics, features)
      3. Code repositories & software
      Desktop tools
      1. Allen Cell & Structure Segmenter
      2. AGAVE 3D pathtrace image viewer
      Web tools
      1. BioFile Finder
      2. Cell Feature Explorer
      3. Integrated Mitotic Stem Cell
      4. └ Z-stack viewer
      5. └ 3D viewer
      Web tools (con't)
      1. Simularium viewer
      2. Timelapse Feature Explorer
      3. Visual Guide to Human Cells
      4. Vol-E (Web Volume Viewer)
      5. 3D Cell Viewer
  • Analysis & Modeling
      Allen Integrated Cell models
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. └ Z-stack viewer
      4. └ 3D viewer
      5. Allen Integrated Cell
      6. └ 3D Probabilistic Modeling
      7. └ Label-free Determination
      4D biology models
      1. Simularium viewer
      Methodologies
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      2. hiPS cells during mitosis
      3. Differentiation into cardiomyocytes
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      5. hiPS cell structure overview
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Cell Catalog quickview

Cell lines currently available for your lab in the Cell Catalog and as image data in the Cell Feature Explorer and 3D Cell Viewer

mEGFP-tagged Sec61 ß
Cell membrane | DNA | Sec61 ß
Tagged protein name: Sec61 ß    
  Primary structure labeled: Endoplasmic Reticulum
  Allen Institute Cell Line ID: AICS-10

mEGFP-tagged Tom20
Cell membrane | DNA | Tom20
Tagged protein name: Tom20    
  Primary structure labeled: Mitochondria
  Allen Institute Cell Line ID: AICS-11

mEGFP-tagged α-tubulin
Cell membrane | DNA | α-tubulin
Tagged protein name: α-tubulin    
  Primary structure labeled: Microtubules
  Allen Institute Cell Line ID: AICS-12

mEGFP-tagged Lamin B1
Cell membrane | DNA | Lamin B1
Tagged protein name: Lamin B1    
  Primary structure labeled: Nuclear envelope
  Allen Institute Cell Line ID: AICS-13

mEGFP-tagged Fibrillarin
Cell membrane | DNA | Fibrillarin
Tagged protein name: Fibrillarin    
  Primary structure labeled: Nucleolus
  Allen Institute Cell Line ID: AICS-14

mEGFP-tagged ß-actin
Cell membrane | DNA | ß-actin
Tagged protein name: ß-actin    
  Primary structure labeled: Filamentous actin
  Allen Institute Cell Line ID: AICS-16

mEGFP-tagged Desmoplakin
Cell membrane | DNA | Desmoplakin
Tagged protein name: Desmoplakin (right panel shows transmitted light overlay)  
  Primary structure labeled: Desmosomes
  Allen Institute Cell Line ID: AICS-17

mEGFP-tagged LAMP-1
Cell membrane | DNA | LAMP-1
Tagged protein name: LAMP-1   
  Primary structure labeled: Lysosome
  Allen Institute Cell Line ID: AICS-22

mEGFP-tagged TJP-Z01
Cell membrane | DNA | TJP-Z01
Tagged protein name: Tight junction protein ZO1    
  Primary structure labeled: Tight junctions
  Allen Institute Cell Line ID: AICS-23

mEGFP-tagged Non-muscle myosin IIB
Cell membrane | DNA | Non-muscle myosin IIB
Tagged protein name: Non-muscle myosin heavy chain IIB    
  Primary structure labeled: Actomyosin bundles
  Allen Institute Cell Line ID: AICS-24

mEGFP-tagged Sialyltransferase 1 (biallelic)
Cell membrane | DNA | Sialyltransferase 1 (biallelic)   
Tagged protein name: Sialyltransferase 1 (biallelic)   
  Primary structure labeled: Golgi
  Allen Institute Cell Line ID: AICS-25, clone 44

mEGFP-tagged Sialyltransferase 1 (monoallelic)
Cell membrane | DNA | Sialyltransferase 1 (monoallelic)   
Tagged protein name: Sialyltransferase 1 (monoallelic)    
  Primary structure labeled: Golgi
  Allen Institute Cell Line ID: AICS-25, clone 1

mTagRFP-T-tagged α-tubulin
Cell membrane | DNA | α-tubulin
Tagged protein name: α-tubulin    
  Primary structure labeled: Microtubules
  Allen Institute Cell Line ID: AICS-31

mTagRFP-T-tagged Centrin-2
Cell membrane | DNA | Centrin-2
Tagged protein name: Centrin-2    
  Primary structure labeled: Centrosome
  Allen Institute Cell Line ID: AICS-32

mEGFP-tagged Troponin I (in cardiomyocytes)
Cell membrane | DNA | Troponin I (in cardiomyocytes)
Tagged protein name: Troponin I, slow skeletal muscle (expressed in cardiomyocytes)
  Primary structure labeled: Sarcomeric thin filament
  Allen Institute Cell Line ID: AICS-37

mEGFP-tagged Rab-5A (biallelic)
Cell membrane | DNA | Rab-5A (biallelic)
Tagged protein name: Ras-related protein Rab-5A (biallelic)
  Primary structure labeled: Endosome
  Allen Institute Cell Line ID: AICS-40, clone 35

mEGFP-tagged Rab-5A (monoallelic)
Cell membrane | DNA | Rab-5A (monoallelic)
Tagged protein name: Ras-related protein Rab-5A (monoallelic)
  Primary structure labeled: Endosome
  Allen Institute Cell Line ID: AICS-40, clone 13

mEGFP-tagged SERCA2
Cell membrane | DNA | SERCA2
Tagged protein name: SERCA2 - expressed in cardiomyocytes
  Primary structure labeled: Sarcoplasmic reticulum
  Allen Institute Cell Line ID: AICS-46

mEGFP-tagged Titin
Cell membrane | DNA | Titin
Tagged protein name: Titin - expressed in cardiomyocytes
  Primary structure labeled: Sarcomere M-line
  Allen Institute Cell Line ID: AICS-48

mEGFP-tagged MLC-2a
Cell membrane | DNA | MLC-2a
Tagged protein name: MLC-2a - expressed in cardiomyocytes
  Primary structure labeled: Sarcomeric thick filaments
  Allen Institute Cell Line ID: AICS-52

mEGFP-tagged Connexin-43
Cell membrane | DNA | Connexin-43
Tagged protein name: Connexin-43
  Primary structure labeled: Gap junction
  Allen Institute Cell Line ID: AICS-53

mEGFP-tagged Nucleophosmin
Cell membrane | DNA | Nucleophosmin
Tagged protein name: Nucleophosmin
  Primary structure labeled: Nucleolus
  Allen Institute Cell Line ID: AICS-57

mEGFP-tagged ß-catenin
Cell membrane | DNA | ß-catenin
Tagged protein name: Beta catenin
  Primary structure labeled: Adherens junctions
  Allen Institute Cell Line ID: AICS-58

mEGFP-tagged H2B type 1-J
Cell membrane | DNA | H2B type 1-J
Tagged protein name: Histone H2B type 1-J
  Primary structure labeled: Histone
  Allen Institute Cell Line ID: AICS-61

mEGFP-tagged Nup153
Cell membrane | DNA | Nup153
Tagged protein name: Nucleoporin Nup153
  Primary structure labeled: Nuclear pores
  Allen Institute Cell Line ID: AICS-62

EGFP-tagged Paxillin (time-series movie, not z-stack)
Tagged protein name: Paxillin 
•not yet available in 3D Cell Viewer
•right panel shows transmitted light overlay
  Primary structure labeled: Cell-matrix adhesions
  Allen Institute Cell Line ID: AICS-5

Cell membrane | DNA | α-actinin
Tagged protein name: α-actinin
•This line not yet available through Coriell
  Primary structure labeled: Actin bundles
  Allen Institute Cell Line ID: AICS-7

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Allen Institute for Cell Science is a part of the Allen Institute. The mission of the Allen Institute is to understand the principles that govern life, and to advance health. Our creative and multi-dimensional teams focus on answering some of the biggest questions in bioscience. We accelerate foundational research, catalyze bold ideas, develop tools and models, and openly share our science to make a broad, transformational impact on the world.
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  • About
      Institute
      1. Our science: CellScapes
      2. Past foundational projects
      3. News feed
      4. About us
      5. Careers
  • Allen Cell Collection
      Order cells & plasmids
      1. Cell Catalog
      2. Disease Collection Cell Catalog
      3. Cell Catalog quickview
      4. Cell video shorts
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
      4. Support forum
      About our hiPS cells
      1. hiPS Cell Structure Overview
      2. Visual Guide to Human Cells
      3. Cell structure observations
      4. Why endogenous tagging?
      5. Differentiation into cardiomyocytes
      6. Genomics
      7. Download cell data: Images, genomics, & features
  • Data & Digital Tools
      General
      1. Tools and resources overview
      2. Download cell data (images, genomics, features)
      3. Code repositories & software
      Desktop tools
      1. Allen Cell & Structure Segmenter
      2. AGAVE 3D pathtrace image viewer
      Web tools
      1. BioFile Finder
      2. Cell Feature Explorer
      3. Integrated Mitotic Stem Cell
      4. └ Z-stack viewer
      5. └ 3D viewer
      Web tools (con't)
      1. Simularium viewer
      2. Timelapse Feature Explorer
      3. Visual Guide to Human Cells
      4. Vol-E (Web Volume Viewer)
      5. 3D Cell Viewer
  • Analysis & Modeling
      Allen Integrated Cell models
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. └ Z-stack viewer
      4. └ 3D viewer
      5. Allen Integrated Cell
      6. └ 3D Probabilistic Modeling
      7. └ Label-free Determination
      4D biology models
      1. Simularium viewer
      Methodologies
      1. Drug perturbation pilot study
      2. hiPS cells during mitosis
      3. Differentiation into cardiomyocytes
  • Publications
      Articles
      1. Publications
      2. Preprints
      Presentations
      1. Talks & posters
  • Education
      Educational resources
      1. All resources
      2. Teaching materials
      Online tools popular with teachers
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. 3D Cell Feature Explorer
      4. 3D Cell Viewer
      5. hiPS cell structure overview
  • Support
      Questions
      1. FAQs
      2. Forum
      Tutorials for digital tools
      1. Video tutorials
      2. Visual Guide tutorial
      3. AGAVE documentation
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
  • 🔍
      SEARCHBAR