The Allen Institute for Cell Science has developed a tool for the prediction of fluorescently labeled structures in live cells solely from 3D brightfield microscopy images. This approach can be used to predict several structures of interest from the same 3D brightfield image, and can potentially be used in applications as diverse as cross-modal image registration, quantification of live cell imaging, and determination of cell state changes. This approach avoids use of fluorescence microscopy, typically used to identify subcellular structures, and the accompanying dyes and proteins are often expensive, time-consuming, and damaging to the cells.
Learn more about this project in our paper Three dimensional cross-modal image inference: label-free methods for subcellular structure prediction.
In addition to hosting booth #639 in the exhibitor hall, researchers from the Allen Institute for Cell Science will present in several venues including:
We look forward to seeing you at the 2017 ASCB | EMBO Meeting in Philadelphia December 2–6!
Five more fluorescently tagged hiPSC lines have been added to the Allen Cell Collection of GFP-tagged molecules. The new lines include 3 new cellular structures, an existing structure with an alternate fluorescent tag, and one structure with both mono- and bi-allelic versions.
Tubulin, labeling the microtubules, is now available with an mTagRFPt tag providing a red tag in addition to the already available mEGFP tagged version.
Centrosomes can now also be visualized with this red mTagRFPt label via the CETN2 line.
The Golgi is available for the first time in both a mono- and bi-allelic version (Golgi- ST6Gal1 –mEGFP – mono-allelic and Golgi- ST6Gal1 –mEGFP – bi-allelic). Please pay special attention when ordering either of these Golgi lines as the AICS number is now qualified with a three-digit clonal extension to help discriminate between the mono- and bi-allelic edits of the same structure.
The final line in this new batch targets the lysosome, with LAMP1 tagged using mEGFP.
These new additions are summarized below:
Dr. Ruwanthi Gunawardane, Director of Stem Cells and Gene Editing at the Allen Institute for Cell Science was hosted by StemCell Technologies to discuss the following topics:
StemCell Technologies mTeSR1 and the Trilineage Differentiation Kit are used in the cell line creation process.
When: August 23, 2017 @ 10am PDT | 1pm EDT | 6pm BST
StemCell Technologies will host a live webinar with Dr. Ruwanthi Gunawardane, Director of Stem Cells and Gene Editing at the Allen Institute for Cell Science.
Join Dr. Gunawardane to discuss the following topics:
Photos and images from the NVIDIA Booth at NVIDIA’s GPU Technology Conference where Allen Institute for Cell Science investigators Dan Toloudis and Greg Johnson showcased visualization and deep learning technologies of the Allen Cell Explorer that use NVIDIA GPUs.
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Read our newsletter announcing the release of the Allen Cell Explorer.
Visit our founder’s website, PaulAllen.com, to read a story from Allen Institute for Cell Science Executive Director, Rick Horwitz, on the launch of the Allen Cell Explorer: Creating a Dynamic Digital Window Into the Human Cell.
Links on the bottom of our article at alleninstitute.org provide access to the interviews and articles written about the release of the Allen Cell Explorer.