Supplementary Video 3
Overview panel of average morphed cells for 25 cellular structures reconstructed throughout the cell and nuclear shape space.
Each row represents one of the 25 cellular structures, indicated by the color bar on the far left. Each column represents Shape Modes 1-8, respectively. Each frame of the video represents one of the nine map points bins (Fig. 2b; in σ units, indicated at top left) along each shape mode and shows the resultant average morphed cells for the cells in that bin (numbers of cells in Supplementary Data 1. For every cell the top view and side view 1 are shown. Heatmaps as in Extended Data Fig. 5.
Scale bars are 5 μm.
Each row represents one of the 25 cellular structures, indicated by the color bar on the far left. Each column represents Shape Modes 1-8, respectively. Each frame of the video represents one of the nine map points bins (Fig. 2b; in σ units, indicated at top left) along each shape mode and shows the resultant average morphed cells for the cells in that bin (numbers of cells in Supplementary Data 1. For every cell the top view and side view 1 are shown. Heatmaps as in Extended Data Fig. 5.
Scale bars are 5 μm.
Supplementary Video 4
Overview panel video of 25 cellular structures in edge and shape-matched non-edge cells.
a. Within each half, either moving left to right (non-edge cells) or right to left (edge cells), two sets of two individual cell examples of the structure segmentation, the result of morphing that cell’s PILR into the combined non-edge + edge mean cell shape, and the resultant average morphed cells, visualized as in Extended Data Fig. 5.
b. PILR-LDA based reconstructions of average morphed cells at five positions (in σ units; each position is a frame inthe video, indicated by the arrowhead) along the non-edge to edge cell LDA axis. Histograms show frequency of cells along the LDA axis within non-edge (black) and edge (red) cell populations. Dotted vertical lines indicate the means. X indicates that LDA axis primarily identified technical differences in the PILR (Supplemental Methods). Heatmaps as in Extended Data Fig. 5.
Scale bars are 5 μm.
a. Within each half, either moving left to right (non-edge cells) or right to left (edge cells), two sets of two individual cell examples of the structure segmentation, the result of morphing that cell’s PILR into the combined non-edge + edge mean cell shape, and the resultant average morphed cells, visualized as in Extended Data Fig. 5.
b. PILR-LDA based reconstructions of average morphed cells at five positions (in σ units; each position is a frame inthe video, indicated by the arrowhead) along the non-edge to edge cell LDA axis. Histograms show frequency of cells along the LDA axis within non-edge (black) and edge (red) cell populations. Dotted vertical lines indicate the means. X indicates that LDA axis primarily identified technical differences in the PILR (Supplemental Methods). Heatmaps as in Extended Data Fig. 5.
Scale bars are 5 μm.
Supplementary Video 5
Overview panel video of 25 cellular structures in early mitosis.
a. Sets of two columns showing example(s) of individual cell structure segmentations and the resulting average morphed cells for all four early mitotic populations (i1, i2, m1, m2) in the appropriate mean mitotic cell shapes, visualized as in Extended Data Fig. 5.
b. PILR-LDA based reconstructions of average morphed cells at five positions (in σ units; each position is a frame inthe video, indicated by the arrowhead) along the i1 to m1 (left) and i2 to m2 (right) LDA axes. Histograms show the frequency of i1 and m1 cells or i2 and m2 cells along the LDA axis. Dotted vertical lines indicate the means. X indicates that LDA axis primarily identified technical differences in the PILR (Supplemental Methods). Heatmaps as in Extended Data Fig. 5.
Scale bars are 5 μm.
a. Sets of two columns showing example(s) of individual cell structure segmentations and the resulting average morphed cells for all four early mitotic populations (i1, i2, m1, m2) in the appropriate mean mitotic cell shapes, visualized as in Extended Data Fig. 5.
b. PILR-LDA based reconstructions of average morphed cells at five positions (in σ units; each position is a frame inthe video, indicated by the arrowhead) along the i1 to m1 (left) and i2 to m2 (right) LDA axes. Histograms show the frequency of i1 and m1 cells or i2 and m2 cells along the LDA axis. Dotted vertical lines indicate the means. X indicates that LDA axis primarily identified technical differences in the PILR (Supplemental Methods). Heatmaps as in Extended Data Fig. 5.
Scale bars are 5 μm.