Cell Shorts
mini-documentaries on the use & impact of hiPS cells in labs around the world

Cell Shorts:
A common platform for human cell research
Bruce Conklin from the Gladstone Institutes created WTC: a widely used human induced pluripotent stem cell line. The Allen Institute for Cell Science uses this line in the Allen Cell Collection: the first publicly available collection of gene edited, fluorescently tagged human induced pluripotent stem cells. See how the Conklin lab is using these gene edited cells to find therapies for life threatening human genetic diseases.
Cell Shorts:
Illuminating the kidney
In our new video series Cell Shorts, see how researcher Beno Freeman and his colleagues at the University of Washington are using the Allen Institute for Cell Science’s publicly available human induced pluripotent stem cells to study kidney disease and regeneration.
The Cell Catalog contains our completed cell lines, which are available through Coriell as part of the Allen Cell Collection. Click a cell line on the first page of the Cell Catalog to opens pages with supplemental material (data, images, videos) related to the rigorous testing and quality control steps each line has undergone.
More videos to come..

Allen Cell Methods
Instructional Videos for Success in the Lab

To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, researchers from our teams highlight nuanced techniques and helpful tips while demonstrating various laboratory protocols.
Read our paper on Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization in Molecular Biology of the Cell (MBoC).
ALLEN CELL METHODS
1. Thawing hiPSCs
Jacqueline Smith highlights nuanced techniques and helpful tips while demonstrating how to thaw a vial of our human gene-edited induced pluripotent stem cells.
ALLEN CELL METHODS
2. Culturing & maintaining hiPSCs
Amanda Haupt, demonstrates our single cell passaging protocol for gene editing human induced pluripotent stem cells.
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This process should take 15–30 minutes to complete and we recommend passaging a maximum of two cell lines at a time in parallel. Researchers can obtain our publicly available gene-edited fluorescently tagged cell lines through our Allen Cell Catalog.
ALLEN CELL METHODS
3. Gene editing: RNP transfection for gene-editing hiPSCs
Maggie Fuqua demonstrates our RNP transfection protocol for gene editing human induced pluripotent stem cells.

​This process should take 30–60 minutes to complete. We recommend preparing all reagents and materials beforehand. Our researchers have used the RNP transfection method for all of the gene-edited hiPS cells available through our Allen Cell Catalog.
JOVE VIDEO JOURNAL
4. Gene editing: Endogenous Protein Tagging in hiPSCs Using CRISPR/Cas9
This Jove Video Journal article presents a protocol we developed for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.

​Access more information detailing our scientific methods & standard operating procedures. Researchers can obtain these hiPS cells or plasmids through our Cell Catalog.
​ALLEN CELL METHODS
5. Cardiomyocyte Differentiation of hiPSCs
Angel Nelson highlights nuanced techniques and helpful tips while demonstrating our cardiac differentiation protocol for the WTC parental line and our gene-edited cells.

If the experiment is successful, you can expect to see beating cardiomyocytes at day 7. Researchers can obtain the hiPS cells used in this protocol through our Allen Cell Catalog.
​ALLEN CELL METHODS
6. Techniques while working with Matrigel
Angel Nelson highlights nuanced techniques and helpful tips while working with Matrigel.

Researchers can obtain the hiPS cells used in this protocol through our Allen Cell Catalog.
​ALLEN CELL METHODS
7. Cryopreservation of clonal human iPS cell lines in 96-well plates
To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, our Amanda Haupt highlights helpful tips while demonstrating how to cryopreserve clonal human induced pluripotent stem cells in 96-well plates.

Researchers can obtain the hiPS cells used in this protocol through our Allen Cell Catalog.
​ALLEN CELL METHODS
8. Automated tissue culture platform
To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, our Mackenzie Coston showcases the automated tissue culture platform we use to seed, passage, feed, and maintain our cells.​

Researchers can obtain the hiPS cells used in this protocol through our Allen Cell Catalog.

Allen Cell Explorer Tutorials
Learn how to use tools & data freely available here on allencell.org

​Allen Cell Tutorials:
Navigating the Cell Catalog
Amanda Haupt represents the gene-editing team to walks you through the cell lines and supporting data available in the Allen Cell Catalog, and how to obtain the lines for use in your labs.
The Cell Catalog contains our completed cell lines, which are available through Coriell as part of the Allen Cell Collection. Click a cell line on the first page of the Cell Catalog to opens pages with supplemental material (data, images, videos) related to the rigorous testing and quality control steps each line has undergone.
​Allen Cell Tutorials:
Navigating the 3D Cell Viewer
​Megan Riel-Mehan represents the Animated Cell group to present an overview of several major upgrades and an easier to use interface for the 3D Cell Viewer tool.

The 3D Cell Viewer is a tool in the Allen Cell Explorer for viewing thousands of 3D images of cellular structures and organelles.
More videos to come..
Allen Institute for Cell Science is a part of the Allen Institute. The mission of the Allen Institute is to understand the principles that govern life, and to advance health. Our creative and multi-dimensional teams focus on answering some of the biggest questions in bioscience. We accelerate foundational research, catalyze bold ideas, develop tools and models, and openly share our science to make a broad, transformational impact on the world.
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