New Webinar Available: Using CRISPR/Cas9 to Model Stem Cell Organization & Dynamics

View Webinar on StemCell.com

Dr. Ruwanthi Gunawardane, Director of Stem Cells and Gene Editing at the Allen Institute for Cell Science was hosted by StemCell Technologies to discuss the following topics:

• Creation of a dynamic visual model of hiPS cell organization through the endogenous labeling of major cellular organelles with a fluorescent protein
• Overview of the CRISPR/Cas9 methodologies used for endogenous gene tagging, screening for precise editing, and cell biological and genomic quality control
• An unprecedented clarity in live cell imaging provided by the endogenous tagging of hiPS cells
• Potential applications of the Allen Institute's hiPS cell lines for basic science and disease modeling
• Her group's recent publication in MBoC: Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization

 
StemCell Technologies mTeSR1 and the Trilineage Differentiation Kit are used in the cell line creation process.