ALLEN CELL EXPLORER
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      3. └ Z-stack viewer
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      6. └ 3D viewer
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      8. └ 3D viewer
      9. Visual Guide to Human Cells
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      5. hiPS cell structure overview
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News Feed

For general news from the Allen Institute for Cell Science, visit alleninstitute.org

Five new cell lines added to the Cell Catalog

10/4/2017

 
Picture
Five more fluorescently tagged hiPSC lines have been added to the Allen Cell Collection of GFP-tagged molecules. The new lines include 3 new cellular structures, an existing structure with an alternate fluorescent tag, and one structure with both mono- and bi-allelic versions.

Tubulin, labeling the microtubules, is now available with an mTagRFPt tag providing a red tag in addition to the already available mEGFP tagged version.

Centrosomes can now also be visualized with this red mTagRFPt label via the CETN2 line.

The Golgi is available for the first time in both a mono- and bi-allelic version (Golgi- ST6Gal1 –mEGFP – mono-allelic and Golgi- ST6Gal1 –mEGFP – bi-allelic). Please pay special attention when ordering either of these Golgi lines as the AICS number is now qualified with a three-digit clonal extension to help discriminate between the mono- and bi-allelic edits of the same structure.

​The final line in this new batch targets the lysosome, with LAMP1 tagged using mEGFP.

These new additions are summarized below:
  • TUBA1b-mTagRFP-T (α tubulin) targeting microtubules
  • Centrin-2–mTagRFP-T targeting centrosomes
  • Sialyltransferase 1–mEGFP targeting Golgi (monoallelic)
  • Sialyltransferase 1–mEGFP targeting Golgi (biallelic)
  • LAMP-1–mEGFP targeting lysosomes

Allen Cell Explorer Tutorial: Navigating the Cell Catalog

10/4/2017

 
Picture
Picture
Check out our first video tutorial on how to use the Allen Cell Explorer. Amanda Haupt walks you through the cell lines and supporting data available in the catalog, and how to obtain the lines for use in your labs.

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  • About
      Institute
      1. News feed
      2. What we do
      3. Publications
      4. Allen Institute | allenInstitute.org
      5. Careers
      Site
      1. Home page
      2. Site updates
      3. Archived content
  • Allen Cell Collection
      Order cells & plasmids
      1. Cell Catalog
      2. Cell Catalog quickview
      3. Cell Shorts (documentaries on labs using our cells)
      4. Support forum
      Lab methods
      1. Instructional videos for success in the lab
      2. Standard operating procedures (written methods)
      3. Illustrated overviews
      About our hiPS cells
      1. hiPS Cell Structure Overview
      2. Visual Guide to Human Cells
      3. Cell structure observations
      4. Why endogenous tagging?
      5. Differentiation into cardiomyocytes
      6. Genomics
      7. Download cell data (images, genomics, features)
  • Data & Digital Tools
      Online image analysis
      1. Cell Feature Explorer (plotting & 3D viewer)
      2. 3D cell viewer (pre2018)
      3. Deep cell zoom (216,016 cells)
      Online modeling viewers
      1. Visual Guide to Human Cells
      2. Simularium (4D visual analysis)
      3. Integrated Mitotic Stem Cell
      4. └ Z-stack viewer
      5. └ 3D viewer
      6. Allen Integrated Cell viewer
      7. Label-free examples viewer
      8. 3D probabilistic model viewer
      Desktop tools
      1. Allen Cell & Structure Segmenter
      2. AGAVE 3D pathtrace image viewer
      Data & code
      1. Download cell data (images, genomics, features)
      2. Code repositories & software
  • Analysis & Modeling
      Allen Integrated Cell models
      1. Overview
      2. Integrated Mitotic Stem Cell
      3. └ Z-stack viewer
      4. └ 3D viewer
      5. Label-free Determination
      6. └ 3D viewer
      7. 3D Probabilistic Modeling
      8. └ 3D viewer
      9. Visual Guide to Human Cells
      4D biology models
      1. Simularium (online 4D viewer)
      Methodologies
      1. Drug perturbation pilot study
      2. hiPS cells during mitosis
      3. Differentiation into cardiomyocytes
  • Publications
      Articles
      1. All journal publications
      2. Preprints (biorxiv, arxiv)
      Posters
      1. Select posters
  • Education
      Education resources
      1. All Resources
      2. Teaching materials
      Online tools popular with teachers
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. Cell Feature Explorer (interactive plotting & 3D viewer)
      4. 3D cell viewer (pre2018 data)
      5. hiPS cell structure overview
  • Support
      Questions
      1. FAQs
      2. Forum
      Tutorials for digital tools
      1. Digital tool tutorials with videos
      2. Visual Guide tutorial
      3. AGAVE user guide
      Lab methods
      1. Instructional videos for success in the lab
      2. Standard operating procedures (written methods)
      3. Illustrated overviews
  • 🔍
      SEARCHBAR