Five more fluorescently tagged hiPSC lines have been added to the Allen Cell Collection of GFP-tagged molecules. The new lines include 3 new cellular structures, an existing structure with an alternate fluorescent tag, and one structure with both mono- and bi-allelic versions.

Tubulin, labeling the microtubules, is now available with an mTagRFPt tag providing a red tag in addition to the already available mEGFP tagged version.

Centrosomes can now also be visualized with this red mTagRFPt label via the CETN2 line.

The Golgi is available for the first time in both a mono- and bi-allelic version (Golgi- ST6Gal1 –mEGFP – mono-allelic and Golgi- ST6Gal1 –mEGFP – bi-allelic). Please pay special attention when ordering either of these Golgi lines as the AICS number is now qualified with a three-digit clonal extension to help discriminate between the mono- and bi-allelic edits of the same structure.

​The final line in this new batch targets the lysosome, with LAMP1 tagged using mEGFP.

These new additions are summarized below:

• TUBA1b-mTagRFP-T (α tubulin) targeting microtubules
• Centrin-2–mTagRFP-T targeting centrosomes
• Sialyltransferase 1–mEGFP targeting Golgi (monoallelic)
• Sialyltransferase 1–mEGFP targeting Golgi (biallelic)
• LAMP-1–mEGFP targeting lysosomes