Five more fluorescently tagged hiPSC lines have been added to the Allen Cell Collection of GFP-tagged molecules. The new lines include 3 new cellular structures, an existing structure with an alternate fluorescent tag, and one structure with both mono- and bi-allelic versions.
Tubulin, labeling the microtubules, is now available with an mTagRFPt tag providing a red tag in addition to the already available mEGFP tagged version.
Centrosomes can now also be visualized with this red mTagRFPt label via the CETN2 line.
The Golgi is available for the first time in both a mono- and bi-allelic version (Golgi- ST6Gal1 –mEGFP – mono-allelic and Golgi- ST6Gal1 –mEGFP – bi-allelic). Please pay special attention when ordering either of these Golgi lines as the AICS number is now qualified with a three-digit clonal extension to help discriminate between the mono- and bi-allelic edits of the same structure.
The final line in this new batch targets the lysosome, with LAMP1 tagged using mEGFP.
These new additions are summarized below:
Check out our first video tutorial on how to use the Allen Cell Explorer. Amanda Haupt walks you through the cell lines and supporting data available in the catalog, and how to obtain the lines for use in your labs.
Dr. Ruwanthi Gunawardane, Director of Stem Cells and Gene Editing at the Allen Institute for Cell Science was hosted by StemCell Technologies to discuss the following topics:
StemCell Technologies mTeSR1 and the Trilineage Differentiation Kit are used in the cell line creation process.
When: August 23, 2017 @ 10am PDT | 1pm EDT | 6pm BST
StemCell Technologies will host a live webinar with Dr. Ruwanthi Gunawardane, Director of Stem Cells and Gene Editing at the Allen Institute for Cell Science.
Join Dr. Gunawardane to discuss the following topics:
Photos and images from the NVIDIA Booth at NVIDIA’s GPU Technology Conference where Allen Institute for Cell Science investigators Dan Toloudis and Greg Johnson showcased visualization and deep learning technologies of the Allen Cell Explorer that use NVIDIA GPUs.
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Read our newsletter announcing the release of the Allen Cell Explorer.
Visit our founder’s website, PaulAllen.com, to read a story from Allen Institute for Cell Science Executive Director, Rick Horwitz, on the launch of the Allen Cell Explorer: Creating a Dynamic Digital Window Into the Human Cell.
Links on the bottom of our article at alleninstitute.org provide access to the interviews and articles written about the release of the Allen Cell Explorer.
The Allen Cell Explorer is a one-of-a-kind portal and dynamic digital window into the human cell. The website combines large-scale 3D imaging data, the first application of deep learning to create predictive models of cell organization, gene edited human stem cell lines and a growing suite of powerful tools.
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