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Extracting multiple cellular structures from single bright-field microscopy images

11/9/2017

 
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The Allen Institute for Cell Science has developed a tool for the prediction of fluorescently labeled structures in live cells solely from 3D brightfield microscopy images. This approach can be used to predict several structures of interest from the same 3D brightfield image, and can potentially be used in applications as diverse as cross-modal image registration, quantification of live cell imaging, and determination of cell state changes. This approach avoids use of fluorescence microscopy, typically used to identify subcellular structures, and the accompanying dyes and proteins are often expensive, time-consuming, and damaging to the cells.
Learn more about this project in our paper Three dimensional cross-modal image inference: label-free methods for subcellular structure prediction.
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cellscience.alleninstitute.org
  • About
    • What We Do
    • FAQs
    • Videos & Tutorials
    • Site Updates
    • < Forum >
    • < AllenInstitute.org >
  • Animated Cell
    • Educational Resources
    • Visual Guide to Human Cells
    • Visual Guide Tutorial
    • Research Projects >
      • Pathtrace rendering
  • Cells & Biology
    • Cell Catalog
    • About our Cells >
      • hiPS Cell Biology Overview
      • Cell Structure Observations
      • Cell Catalog QuickView
    • Genomics
    • Methods & SOPs >
      • Methods for Cells in the Lab
      • Cell Methods: Videos from the Lab
      • Methods for Microscopy
    • Research Projects >
      • hiPS Cells During Mitosis
      • Drug Perturbation Pilot
      • Differentiation Into Cardiomyocytes
      • Why Endogenous Tagging?
  • Data & Tools
    • Cell Catalog
    • 3D Cell Viewer
    • Cell Feature Explorer
    • Integrated Mitotic Stem Cell Z-stack Viewer
    • Deep Cell Zoom
    • Segmenter
    • Data Downloading
    • Software & Code
    • Simularium
    • Research Projects >
      • Extracting Information
  • Modeling & Analysis
    • Integrated Mitotic Stem Cell
    • Allen Integrated Cell
    • Label-free Determination
    • Data Notebook Exploration >
      • Cell Shape Analysis
      • Programmatic Data Access
    • Research Projects >
      • 3D Probabilistic Modeling
  • News & Publications
    • News Feed
    • Publications
    • Archived Content