In addition to hosting a VR cell tour at booth #311 in the exhibitor hall, researchers from the Allen Institute for Cell Science will present in several venues including:

• a Platform Session on Computational Methods and Bioinformatics
• in the Cell Biophysics Subgroup
• an exhibitor session
• and in front of 3 posters

​We look forward to seeing you at BPS!

Interphase and mitotic cell neighbors, imaged by our microscopes, enhanced by deep learning, and imagined by our illustrators to be enjoying a festive Washington winter.

We have released a new tutorial video. The 3D Cell Viewer is a tool in the Allen Cell Explorer for viewing thousands of 3D images of cellular structures and organelles.

​Megan Riel-Mehan represents the Animated Cell group to present an overview of several major upgrades and an easier to use interface for the 3D Cell Viewer tool.

The Allen Institute for Cell Science has developed a tool for the prediction of fluorescently labeled structures in live cells solely from 3D brightfield microscopy images. This approach can be used to predict several structures of interest from the same 3D brightfield image, and can potentially be used in applications as diverse as cross-modal image registration, quantification of live cell imaging, and determination of cell state changes. This approach avoids use of fluorescence microscopy, typically used to identify subcellular structures, and the accompanying dyes and proteins are often expensive, time-consuming, and damaging to the cells.

Learn more about this project in our paper Three dimensional cross-modal image inference: label-free methods for subcellular structure prediction.

In addition to hosting booth #639 in the exhibitor hall, researchers from the Allen Institute for Cell Science will present in several venues including:

• 3 Special Interest Subgroup Meetings
• a Tech Talk in the exhibitor hall
• at Minisumposium (#6)
• a virtual reality (VR) tour of our cells at booth #639
• and in front of 5 posters

​We look forward to seeing you at the 2017 ASCB | EMBO Meeting​ in Philadelphia December 2–6!

Five more fluorescently tagged hiPSC lines have been added to the Allen Cell Collection of GFP-tagged molecules. The new lines include 3 new cellular structures, an existing structure with an alternate fluorescent tag, and one structure with both mono- and bi-allelic versions.

Tubulin, labeling the microtubules, is now available with an mTagRFPt tag providing a red tag in addition to the already available mEGFP tagged version.

Centrosomes can now also be visualized with this red mTagRFPt label via the CETN2 line.

The Golgi is available for the first time in both a mono- and bi-allelic version (Golgi- ST6Gal1 –mEGFP – mono-allelic and Golgi- ST6Gal1 –mEGFP – bi-allelic). Please pay special attention when ordering either of these Golgi lines as the AICS number is now qualified with a three-digit clonal extension to help discriminate between the mono- and bi-allelic edits of the same structure.

​The final line in this new batch targets the lysosome, with LAMP1 tagged using mEGFP.

These new additions are summarized below:

• TUBA1b-mTagRFP-T (α tubulin) targeting microtubules
• Centrin-2–mTagRFP-T targeting centrosomes
• Sialyltransferase 1–mEGFP targeting Golgi (monoallelic)
• Sialyltransferase 1–mEGFP targeting Golgi (biallelic)
• LAMP-1–mEGFP targeting lysosomes

Dr. Ruwanthi Gunawardane, Director of Stem Cells and Gene Editing at the Allen Institute for Cell Science was hosted by StemCell Technologies to discuss the following topics:

• Creation of a dynamic visual model of hiPS cell organization through the endogenous labeling of major cellular organelles with a fluorescent protein
• Overview of the CRISPR/Cas9 methodologies used for endogenous gene tagging, screening for precise editing, and cell biological and genomic quality control
• An unprecedented clarity in live cell imaging provided by the endogenous tagging of hiPS cells
• Potential applications of the Allen Institute's hiPS cell lines for basic science and disease modeling
• Her group's recent publication in MBoC: Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization

 
StemCell Technologies mTeSR1 and the Trilineage Differentiation Kit are used in the cell line creation process.