 Our new Jove Video Journal article presents a protocol we developed for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.
The Allen Cell Collection now contains five new fluorescently tagged stem cell lines, including the first cells in the collection with a tag specific to heart muscle cells, or cardiomyocytes. To date, all the cell lines have been gene edited to carry a fluorescent marker that is produced in undifferentiated cells, before they go on to become specific cell types, like cardiomyocytes, nerve cells or liver cells.
 To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, our Angel Nelson highlights nuanced techniques and helpful tips while demonstrating our cardiac differentiation protocol for the WTC parental line and our gene-edited cells. If the experiment is successful, you can expect to see beating cardiomyocytes at day 7.
The Allen Institute for Cell Science today launched the Allen Integrated Cell, the world's first predictive and comprehensive 3D model of a live human cell. This new visualization of a large collection of human stem cells will allow researchers around the world to see structures inside a living cell together at the same time, providing a baseline for better understanding healthy cells and for studying human disease models. Building off last year's launch of the Allen Cell Explorer, the Allen Integrated Cell comprises two different models that can predict the shape and location of cellular structures. The first, a probabilistic model, emerged from a machine learning approach that accurately predicts the most probable shape and location of structures in a cell based solely on the shape of that cell's plasma membrane and nucleus. 
 We have released a new Instructional Video. To ensure researchers have success working with our cell lines, our Maggie Fuqua demonstrates our RNP transfection protocol for gene editing hiPSCs.  Please come see us at Biophysical Society's Annual Meeting in San Francisco February 17-21!2/14/2018
 In addition to hosting a VR cell tour at booth #311 in the exhibitor hall, researchers from the Allen Institute for Cell Science will present in several venues including:
1st video in a new series of Allen Cell Methods released: Single cell passaging human iPS cells1/11/2018
 We have release the first in a new series of Instructional Videos! To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, our Amanda Haupt highlights nuanced techniques and helpful tips while demonstrating our single cell passaging protocol. This process should take between fifteen and thirty minutes to complete and we recommend passaging a maximum of two cell lines at a time in parallel. Researchers can obtain our publicly available gene-edited fluorescently tagged cell lines through our Allen Cell Catalog.   Interphase and mitotic cell neighbors, imaged by our microscopes, enhanced by deep learning, and imagined by our illustrators to be enjoying a festive Washington winter. Happy Holidays from all of us at the Allen Institute for Cell Science
 We have released a new tutorial video. The 3D Cell Viewer is a tool in the Allen Cell Explorer for viewing thousands of 3D images of cellular structures and organelles. Megan Riel-Mehan represents the Animated Cell group to present an overview of several major upgrades and an easier to use interface for the 3D Cell Viewer tool.  |
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