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Z-stack with overlay Low magnification timelapse Figure. Movies of desmoplakin in desmosomes. Top: Z-stack of live hiPS cells expressing mEGFP-tagged desmoplakin imaged on a spinning-disk confocal microscope. Images start from the bottom of the cells and end at the top. The right panel shows the left panel overlaid onto the equivalent transmitted light image. Bottom: timelapse movie of a hiPS cell colony expressing mEGFP-tagged desmoplakin. Images were collected in 3D every 4 minutes for 8 hours on a spinning-disk confocal microscope. Images are maximum intensity projections; playback speed is 2400x real time.
Observations
Figure. Movies of paxillin in cell-substrate adhesions. Timelapse movies of hiPS cells expressing EGFP-tagged paxillin imaged on a spinning-disk confocal microscope. Left: images were collected as a partial z-stack near the bottom of the cell every 5 minutes for 160 minutes. Image is a maximum intensity projection and movie is sped up 1500x over real time. Right: single slice images near the bottom of the cell taken every 5 minutes for 400 minutes; playback speed is 3000x real time.
Observations
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AboutObservations and descriptions from the microscope Archives
February 2019
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Allen Institute for Cell Science is a part of the Allen Institute. The mission of the Allen Institute is to understand the principles that govern life, and to advance health. Our creative and multi-dimensional teams focus on answering some of the biggest questions in bioscience. We accelerate foundational research, catalyze bold ideas, develop tools and models, and openly share our science to make a broad, transformational impact on the world.
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