Figures. Z-stack and timelapse movies of lysosomes. A) Z-stack of live hiPS cells expressing mEGFP tagged LAMP1 imaged on a spinning-disk confocal microscope. Movie starts at the bottom of the cells and ends at the top. Scale bar = 5µm. B–C) Timelapse movie of live hiPS cells expressing mEGFP-tagged LAMP1. Images were taken as a single slice near the top (B) or bottom (C) of the cell every 1 sec for 100 sec on a spinning-disk confocal microscope. Images have had the ‘despeckle’ ImageJ filter applied. Movie sped up 10x real time. Scale bar = 5 µm.
Observations and descriptions from the microscope