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Cell structure observations

Observations about microscopy videos for 16 of the cell lines available in our Cell Catalog & 3D Cell Viewer.

Chromatin visualized via SMC protein 1A

2/20/2019

 
Movie. High magnification z-stack of a live hiPS cell colony expressing mEGFP-tagged SMC protein 1A. Cells were imaged in 3D on a spinning-disk confocal microscope. Movie starts at the bottom of the cells and ends at the top. Scale bar, 5µm.
Movie. Time-lapse in high magnification movie of a live hiPS cell colony expressing mEGFP-tagged SMC protein 1A. Cells were imaged in 3D on a spinning-disk confocal microscope every 3 min. A single mid-level plane is shown. Frames were histogram matched to adjust for photobleaching. Movie plays at 900x real time. Scale bar, 5 µm.
Movie. Time-lapse in low magnification movie of a live hiPS cell colony expressing mEGFP-tagged SMC protein 1A. Cells were imaged in 3D on a spinning-disk confocal microscope every 5 min. A single mid-level plane is shown. Movie plays at 3000x real time. Scale bar, 20 µm.
Observations
  • SMC protein 1A is encoded by the X-linked gene SMC1A and escapes X-chromosome inactivation.
  • SMC protein 1A is part of the cohesin complex. Cohesin is important for controlling chromosomal shape and organization in interphase and mitosis. Cohesin is best known for its role in joining sister chromatids during part of the cell cycle between DNA replication and anaphase so that that chromatids are properly distributed between daughter cells.
  • In hiPS cells, SMC protein 1A has a granular appearance throughout the nucleus and is largely excluded from the nucleolus. SMC protein 1A reorganizes during cell division, forming puncta that align at the center of the spindle (consistent with localization to centromeres), localizing in a diffuse haze at anaphase, and reappearing in a granular distribution in the nucleus as the nucleus is reassembled. 

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  • About
      Institute
      1. Our science: CellScapes
      2. Past foundational projects
      3. News feed
      4. About us
      5. Careers
  • Allen Cell Collection
      Order cells & plasmids
      1. Cell Catalog
      2. Disease Collection Cell Catalog
      3. Cell Catalog quickview
      4. Cell video shorts
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
      4. Support forum
      About our hiPS cells
      1. hiPS Cell Structure Overview
      2. Visual Guide to Human Cells
      3. Cell structure observations
      4. Why endogenous tagging?
      5. Differentiation into cardiomyocytes
      6. Genomics
      7. Download cell data: Images, genomics, & features
  • Data & Digital Tools
      General
      1. Tools & resources overview
      2. Download cell data (images, genomics, features)
      3. Code repositories & software
      Desktop tools
      1. Allen Cell & Structure Segmenter
      2. AGAVE 3D pathtrace image viewer
      Web tools
      1. BioFile Finder
      2. Cell Feature Explorer
      3. Integrated Mitotic Stem Cell
      4. └ Z-stack viewer
      5. └ 3D viewer
      Web tools (con't)
      1. Simularium viewer
      2. Timelapse Feature Explorer
      3. Visual Guide to Human Cells
      4. Vol-E (Web Volume Viewer)
      5. 3D Cell Viewer
  • Analysis & Modeling
      Allen Integrated Cell models
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. └ Z-stack viewer
      4. └ 3D viewer
      5. Allen Integrated Cell
      6. └ 3D Probabilistic Modeling
      7. └ Label-free Determination
      4D biology models
      1. Simularium viewer
      Methodologies
      1. Drug perturbation pilot study
      2. hiPS cells during mitosis
      3. Differentiation into cardiomyocytes
  • Publications
      Articles
      1. Publications
      2. Preprints
      Presentations
      1. Talks & posters
  • Education
      Educational resources
      1. All resources
      2. Teaching materials
      Online tools popular with teachers
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. 3D Cell Feature Explorer
      4. 3D Cell Viewer
      5. hiPS cell structure overview
  • Support
      Questions
      1. FAQs
      2. Forum
      Tutorials for digital tools
      1. Video tutorials
      2. Visual Guide tutorial
      3. AGAVE documentation
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
  • 🔍
      SEARCHBAR