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Cell structure observations

Observations about microscopy videos for 16 of the cell lines available in our Cell Catalog & 3D Cell Viewer.

Cell-substrate adhesions via Paxillin

3/17/2017

 
High magnification edge of colony (with overlay)
Low magnification of moving colony
Figure. Movies of paxillin in cell-substrate adhesions. Timelapse movies of hiPS cells expressing EGFP-tagged paxillin imaged on a spinning-disk confocal microscope. Left: images were collected as a partial z-stack near the bottom of the cell every 5 minutes for 160 minutes. Image is a maximum intensity projection and movie is sped up 1500x over real time. Right: single slice images near the bottom of the cell taken every 5 minutes for 400 minutes; playback speed is 3000x real time.

Observations
  • Paxillin is a signaling adaptor that is present in most integrin-mediated sites of adhesions between the cell and the extracellular matrix.
  • In hiPS cell colonies, paxillin primarily localizes to small adhesions which form puncta at the bottom of cells that are less than 0.5 microns in diameter. These adhesions are dynamic, forming and turning over as the edge of the cell protrudes and retracts. Larger, elongated adhesions are also present in these cells particularly along the more protrusive edges of colonies.

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  • About
      Institute
      1. Our science: CellScapes
      2. Past foundational projects
      3. News feed
      4. About us
      5. Careers
  • Allen Cell Collection
      Order cells & plasmids
      1. Cell Catalog
      2. Disease Collection Cell Catalog
      3. Cell Catalog quickview
      4. Cell video shorts
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
      4. Support forum
      About our hiPS cells
      1. hiPS Cell Structure Overview
      2. Visual Guide to Human Cells
      3. Cell structure observations
      4. Why endogenous tagging?
      5. Differentiation into cardiomyocytes
      6. Genomics
      7. Download cell data: Images, genomics, & features
  • Data & Digital Tools
      General
      1. Tools & resources overview
      2. Download cell data (images, genomics, features)
      3. Code repositories & software
      Desktop tools
      1. Allen Cell & Structure Segmenter
      2. AGAVE 3D pathtrace image viewer
      Web tools
      1. BioFile Finder
      2. Cell Feature Explorer
      3. Integrated Mitotic Stem Cell
      4. └ Z-stack viewer
      5. └ 3D viewer
      Web tools (con't)
      1. Simularium viewer
      2. Timelapse Feature Explorer
      3. Visual Guide to Human Cells
      4. Vol-E (Web Volume Viewer)
      5. 3D Cell Viewer
  • Analysis & Modeling
      Allen Integrated Cell models
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. └ Z-stack viewer
      4. └ 3D viewer
      5. Allen Integrated Cell
      6. └ 3D Probabilistic Modeling
      7. └ Label-free Determination
      4D biology models
      1. Simularium viewer
      Methodologies
      1. Drug perturbation pilot study
      2. hiPS cells during mitosis
      3. Differentiation into cardiomyocytes
  • Publications
      Articles
      1. Publications
      2. Preprints
      Presentations
      1. Talks & posters
  • Education
      Educational resources
      1. All resources
      2. Teaching materials
      Online tools popular with teachers
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. 3D Cell Feature Explorer
      4. 3D Cell Viewer
      5. hiPS cell structure overview
  • Support
      Questions
      1. FAQs
      2. Forum
      Tutorials for digital tools
      1. Video tutorials
      2. Visual Guide tutorial
      3. AGAVE documentation
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
  • 🔍
      SEARCHBAR