Allen Cell Explorer
  • About
    • What We Do
    • FAQs
    • Videos & Tutorials
    • Site Updates
    • < Forum >
    • < AllenInstitute.org >
  • Animated Cell
    • Educational Resources
    • Visual Guide to Human Cells
    • Visual Guide Tutorial
    • Research Projects >
      • Pathtrace rendering
  • Cells & Biology
    • Cell Catalog
    • About our Cells >
      • hiPS Cell Biology Overview
      • Cell Structure Observations
      • Cell Catalog QuickView
    • Genomics
    • Methods & SOPs >
      • Methods for Cells in the Lab
      • Cell Methods: Videos from the Lab
      • Methods for Microscopy
    • Research Projects >
      • hiPS Cells During Mitosis
      • Drug Perturbation Pilot
      • Differentiation Into Cardiomyocytes
      • Why Endogenous Tagging?
  • Data & Tools
    • Cell Catalog
    • 3D Cell Viewer
    • Cell Feature Explorer
    • Integrated Mitotic Stem Cell Z-stack Viewer
    • Deep Cell Zoom
    • Segmenter
    • Data Downloading
    • Software & Code
    • Simularium
    • Research Projects >
      • Extracting Information
  • Modeling & Analysis
    • Integrated Mitotic Stem Cell
    • Allen Integrated Cell
    • Label-free Determination
    • Data Notebook Exploration >
      • Cell Shape Analysis
      • Programmatic Data Access
    • Research Projects >
      • 3D Probabilistic Modeling
  • News & Publications
    • News Feed
    • Publications
    • Archived Content

Cell Structure Observations

Observations about microscopy videos for each of the 16 cell lines available in our Cell Catalog & 3D Cell Viewer.
Back to About our Cells

Nuclear pores visualized via Nucleoporin Nup153

8/1/2018

 
Movie. Z-stack of live hiPS cell colony expressing mEGFP-tagged Nucleoporin Nup153 protein. Cells were imaged in 3D on a spinning-disk confocal microscope. Movie starts at the bottom of the cells and ends at the top. Scale bar, 5µm.
Movie. Time-lapse movie of live hiPS cell colony expressing mEGFP-tagged Nucleoporin Nup153 protein. Cells were imaged in a 3D volume spanning ~1.5 µm through the middle of the cells on a spinning-disk confocal microscope every 3 min. A single mid-level z-section is shown. Movie plays at 1800x real time. Scale bar, 5 µm.
Observations
  • Nucleoporin Nup153 is a protein within the nuclear pore complex (NPC). The NPC bridges the double membrane of the nuclear envelope to regulate the exchange of molecules between the nucleus and cytoplasm. Nucleoporin Nup153 is located on the nuclear side of the NPC. It is critically involved in the ability of the NPC to transport molecules between the nucleus and cytoplasm; but it also plays other roles in nuclear properties and functions, including the shape of the NPC and the nucleus, and regulation of transcription.
  • In hiPS cells, Nucleoporin Nup153 appears as numerous dense puncta along the nuclear envelope, including within invaginations.
  • During cell division, Nucleoporin Nup153 localization changes with the reorganization of the nuclear envelope. During mitosis, Nuceloporin Nup153 localizes in a dim wavy structure, likely the ER. At the end of mitosis, Nucleoporin Nup153 appears on chromosomes before finally reappearing at the nuclear envelope.

    About

    Observations and descriptions from the microscope

    Archives

    February 2019
    August 2018
    April 2018
    October 2017
    April 2017
    March 2017

    Categories

    All
    3-channel
    3D Rotation
    Actin
    Actinomyosin
    Actin Structures
    Adhesions
    Cardiomyocyte
    Cell Cell Contacts
    Cell-cell Contacts
    Cell Contacts
    Cell Division
    Cell Membrane
    Centrin
    Centrioles
    Chromatin
    Colony
    Connexin
    Connexin-43
    Desmoplakin
    Desmosomes
    DNA
    Endoplasmic Reticulum
    Endosome
    ER
    Fibrillarin
    FUS
    Gap Junctions
    Golgi Apparatus
    H2B
    Histone
    K-Ras
    Lamin
    Lamin B1
    LAMP1
    Lysosomes
    MEGFP
    Membrane
    Microtubule
    Mitochondria
    Mitosis
    MLC-2a
    MLC 2v
    MTagRFP-T
    Muscle
    Myosin
    Nuclear
    Nuclear Envelope
    Nuclear Pores
    Nucleolus
    Nucleophosmin
    Nucleoporin
    Nucleus
    Nup153
    Paraspeckles
    Paxillin
    Peroxisome
    Plasma Membrane
    PMP34
    Rab-5A
    Ras
    RNA-binding Protein
    Sarcomere
    Sarcomere M-line
    Sarcomere Thick Filaments
    Sarcoplasmic Reticulum
    Sec61
    SERCA2
    Sialyltransferase 1
    SMC 1A
    ß-actin
    ST6GAL1
    Stress Granules
    Tight Junctions
    Time Lapse
    Time Series
    Titin
    Tom20
    Troponin
    Tubulin
    ZO1
    Z Stack

    RSS Feed

Home

Terms of Use

Citation Policy

Privacy Policy

FAQs

Help

Send us a message

Copyright © 2020 Allen Institute. All Rights Reserved.
cellscience.alleninstitute.org
  • About
    • What We Do
    • FAQs
    • Videos & Tutorials
    • Site Updates
    • < Forum >
    • < AllenInstitute.org >
  • Animated Cell
    • Educational Resources
    • Visual Guide to Human Cells
    • Visual Guide Tutorial
    • Research Projects >
      • Pathtrace rendering
  • Cells & Biology
    • Cell Catalog
    • About our Cells >
      • hiPS Cell Biology Overview
      • Cell Structure Observations
      • Cell Catalog QuickView
    • Genomics
    • Methods & SOPs >
      • Methods for Cells in the Lab
      • Cell Methods: Videos from the Lab
      • Methods for Microscopy
    • Research Projects >
      • hiPS Cells During Mitosis
      • Drug Perturbation Pilot
      • Differentiation Into Cardiomyocytes
      • Why Endogenous Tagging?
  • Data & Tools
    • Cell Catalog
    • 3D Cell Viewer
    • Cell Feature Explorer
    • Integrated Mitotic Stem Cell Z-stack Viewer
    • Deep Cell Zoom
    • Segmenter
    • Data Downloading
    • Software & Code
    • Simularium
    • Research Projects >
      • Extracting Information
  • Modeling & Analysis
    • Integrated Mitotic Stem Cell
    • Allen Integrated Cell
    • Label-free Determination
    • Data Notebook Exploration >
      • Cell Shape Analysis
      • Programmatic Data Access
    • Research Projects >
      • 3D Probabilistic Modeling
  • News & Publications
    • News Feed
    • Publications
    • Archived Content