Movie. Time-lapse movie of live beating hiPSC-derived cardiomyocytes expressing mEGFP-tagged SERCA2 protein. Twelve days after the onset of differentiation, cells were plated on PEI and laminin coated glass and imaged on a spinning disk confocal microscope 8 days later (20 days total after the onset of differentiation). A single plane of cells was imaged continuously with a 100 ms exposure time. Inset shows detail of SERCA2 in longitudinal sarcoplasmic reticulum (SR). Scale bars, 10 µm. Movie plays in real time.
Observations and descriptions from the microscope