ALLEN CELL EXPLORER
  • About
      Institute
      1. Our science: CellScapes
      2. Past foundational projects
      3. News feed
      4. About us
      5. Careers
  • Allen Cell Collection
      Order cells & plasmids
      1. Cell Catalog
      2. Disease Collection Cell Catalog
      3. Cell Catalog quickview
      4. Cell video shorts
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
      4. Support forum
      About our hiPS cells
      1. hiPS Cell Structure Overview
      2. Visual Guide to Human Cells
      3. Cell structure observations
      4. Why endogenous tagging?
      5. Differentiation into cardiomyocytes
      6. Genomics
      7. Download cell data: Images, genomics, & features
  • Data & Digital Tools
      General
      1. Tools & resources overview
      2. Download cell data (images, genomics, features)
      3. Code repositories & software
      Desktop tools
      1. Allen Cell & Structure Segmenter
      2. AGAVE 3D pathtrace image viewer
      Web tools
      1. BioFile Finder
      2. Cell Feature Explorer
      3. Integrated Mitotic Stem Cell
      4. └ Z-stack viewer
      5. └ 3D viewer
      Web tools (con't)
      1. Simularium viewer
      2. Timelapse Feature Explorer
      3. Visual Guide to Human Cells
      4. Vol-E (Web Volume Viewer)
      5. 3D Cell Viewer
  • Analysis & Modeling
      Allen Integrated Cell models
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. └ Z-stack viewer
      4. └ 3D viewer
      5. Allen Integrated Cell
      6. └ 3D Probabilistic Modeling
      7. └ Label-free Determination
      4D biology models
      1. Simularium viewer
      Methodologies
      1. Drug perturbation pilot study
      2. hiPS cells during mitosis
      3. Differentiation into cardiomyocytes
  • Publications
      Articles
      1. Publications
      2. Preprints
      Presentations
      1. Talks & posters
  • Education
      Educational resources
      1. All resources
      2. Teaching materials
      Online tools popular with teachers
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. 3D Cell Feature Explorer
      4. 3D Cell Viewer
      5. hiPS cell structure overview
  • Support
      Questions
      1. FAQs
      2. Forum
      Tutorials for digital tools
      1. Video tutorials
      2. Visual Guide tutorial
      3. AGAVE documentation
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
  • 🔍
      SEARCHBAR

Cell structure observations

Observations about microscopy videos for 16 of the cell lines available in our Cell Catalog & 3D Cell Viewer.

Sarcoplasmic reticulum visualized via SERCA2

8/1/2018

 
Movie. ​Z-stack of live hiPS cell colony expressing mEGFP-tagged SERCA2 protein. Cells were imaged in 3D on a spinning-disk confocal microscope. Movie starts at the bottom of the cells and ends at the top. Scale bar, 5µm.
​​Movies. Z-stack of live hiPSC-derived cardiomyocytes expressing mEGFP-tagged SERCA2 protein. Twelve days after the onset of differentiation, cells were plated on PEI and laminin coated glass and imaged in 3D on a spinning disk confocal microscope 29 days later (41 days total after the onset of differentiation). Cells were treated with 15 mM of the myosin inhibitor 2,3-Butanedione monoxime (BDM) to prevent beating during image acquisition. Movie starts at the bottom of the cells and ends at the top. Inset shows detail of SERCA2 in longitudinal sarcoplasmic reticulum (SR). Scale bars, 10 µm.
Movie. Time-lapse movie of live beating hiPSC-derived cardiomyocytes expressing mEGFP-tagged SERCA2 protein. Twelve days after the onset of differentiation, cells were plated on PEI and laminin coated glass and imaged on a spinning disk confocal microscope 8 days later (20 days total after the onset of differentiation). A single plane of cells was imaged continuously with a 100 ms exposure time. Inset shows detail of SERCA2 in longitudinal sarcoplasmic reticulum (SR). Scale bars, 10 µm. Movie plays in real time.
Observations
  • SERCA2 (sarco/endoplasmic reticulum calcium ATPase) proteins form pumps that use the energy from ATP hydrolysis to import calcium from the cytosol into the endoplasmic and/or sarcoplasmic reticula (ER and/or SR). This regulates cytosolic calcium concentration, important for many functions, including contractility in cardiomyocytes.
  • In hiPSC, mEGFP-taged SERCA2 localizes to the nuclear periphery and to ER tubules. In contrast, while both mEGFP-tagged SERCA2 and Sec61-beta proteins localize to the ER membrane, Sec61 beta localizes to the nuclear periphery as well as both ER tubules and sheets. This difference in localization may reflect a preference for SERCA2 to localize to smooth ER over rough ER.
  • In hiPSC-derived cardiomyocytes, mEGFP-tagged SERCA2 localizes to the nuclear periphery and to a tubular network throughout the rest of the cytoplasm. The linear alignment of SERCA2 labeling along presumable myofilaments suggests localization to longitudinal SR.
  • mEGFP-tagged SERCA2 moves when cardiomyocytes beat, presumably moving along with the contraction and buckling of myofilaments.

Comments are closed.

    About

    Observations and descriptions from the microscope

    Archives

    February 2019
    August 2018
    April 2018
    October 2017
    April 2017
    March 2017

    Categories

    All
    3-channel
    3D Rotation
    Actin
    Actinomyosin
    Actin Structures
    Adhesions
    Cardiomyocyte
    Cell Cell Contacts
    Cell-cell Contacts
    Cell Contacts
    Cell Division
    Cell Membrane
    Centrin
    Centrioles
    Chromatin
    Colony
    Connexin
    Connexin-43
    Desmoplakin
    Desmosomes
    DNA
    Endoplasmic Reticulum
    Endosome
    ER
    Fibrillarin
    FUS
    Gap Junctions
    Golgi Apparatus
    H2B
    Histone
    K-Ras
    Lamin
    Lamin B1
    LAMP1
    Lysosomes
    MEGFP
    Membrane
    Microtubule
    Mitochondria
    Mitosis
    MLC-2a
    MLC 2v
    MTagRFP-T
    Muscle
    Myosin
    Nuclear
    Nuclear Envelope
    Nuclear Pores
    Nucleolus
    Nucleophosmin
    Nucleoporin
    Nucleus
    Nup153
    Paraspeckles
    Paxillin
    Peroxisome
    Plasma Membrane
    PMP34
    Rab-5A
    Ras
    RNA-binding Protein
    Sarcomere
    Sarcomere M-line
    Sarcomere Thick Filaments
    Sarcoplasmic Reticulum
    Sec61
    SERCA2
    Sialyltransferase 1
    SMC 1A
    ß-actin
    ST6GAL1
    Stress Granules
    Tight Junctions
    Time Lapse
    Time Series
    Titin
    Tom20
    Troponin
    Tubulin
    ZO1
    Z Stack

    RSS Feed

The Institute

Home
Careers
Culture & Community
Archived Content

Legal

Terms of Use
Citation Policy
Privacy Policy
Cookie Settings

Help & contact

​FAQs
Help
​Send us a Message
​Sign up for our Newsletter
Allen Institute for Cell Science is a part of the Allen Institute. The mission of the Allen Institute is to understand the principles that govern life, and to advance health. Our creative and multi-dimensional teams focus on answering some of the biggest questions in bioscience. We accelerate foundational research, catalyze bold ideas, develop tools and models, and openly share our science to make a broad, transformational impact on the world.
Follow Us  
​Copyright © 2025 Allen Institute. All Rights Reserved.
  • About
      Institute
      1. Our science: CellScapes
      2. Past foundational projects
      3. News feed
      4. About us
      5. Careers
  • Allen Cell Collection
      Order cells & plasmids
      1. Cell Catalog
      2. Disease Collection Cell Catalog
      3. Cell Catalog quickview
      4. Cell video shorts
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
      4. Support forum
      About our hiPS cells
      1. hiPS Cell Structure Overview
      2. Visual Guide to Human Cells
      3. Cell structure observations
      4. Why endogenous tagging?
      5. Differentiation into cardiomyocytes
      6. Genomics
      7. Download cell data: Images, genomics, & features
  • Data & Digital Tools
      General
      1. Tools & resources overview
      2. Download cell data (images, genomics, features)
      3. Code repositories & software
      Desktop tools
      1. Allen Cell & Structure Segmenter
      2. AGAVE 3D pathtrace image viewer
      Web tools
      1. BioFile Finder
      2. Cell Feature Explorer
      3. Integrated Mitotic Stem Cell
      4. └ Z-stack viewer
      5. └ 3D viewer
      Web tools (con't)
      1. Simularium viewer
      2. Timelapse Feature Explorer
      3. Visual Guide to Human Cells
      4. Vol-E (Web Volume Viewer)
      5. 3D Cell Viewer
  • Analysis & Modeling
      Allen Integrated Cell models
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. └ Z-stack viewer
      4. └ 3D viewer
      5. Allen Integrated Cell
      6. └ 3D Probabilistic Modeling
      7. └ Label-free Determination
      4D biology models
      1. Simularium viewer
      Methodologies
      1. Drug perturbation pilot study
      2. hiPS cells during mitosis
      3. Differentiation into cardiomyocytes
  • Publications
      Articles
      1. Publications
      2. Preprints
      Presentations
      1. Talks & posters
  • Education
      Educational resources
      1. All resources
      2. Teaching materials
      Online tools popular with teachers
      1. Visual Guide to Human Cells
      2. Integrated Mitotic Stem Cell
      3. 3D Cell Feature Explorer
      4. 3D Cell Viewer
      5. hiPS cell structure overview
  • Support
      Questions
      1. FAQs
      2. Forum
      Tutorials for digital tools
      1. Video tutorials
      2. Visual Guide tutorial
      3. AGAVE documentation
      Lab methods
      1. Video protocols
      2. Written protocols
      3. Our methodology
  • 🔍
      SEARCHBAR