Allen Cell Explorer
  • About
    • What We Do
    • FAQs
    • Videos & Tutorials
    • Site Updates
    • < Forum >
    • < AllenInstitute.org >
  • Animated Cell
    • Educational Resources
    • Visual Guide to Human Cells
    • Visual Guide Tutorial
    • Research Projects >
      • Pathtrace rendering
  • Cells & Biology
    • Cell Catalog
    • About our Cells >
      • hiPS Cell Biology Overview
      • Cell Structure Observations
      • Cell Catalog QuickView
    • Genomics
    • Methods & SOPs >
      • Methods for Cells in the Lab
      • Cell Methods: Videos from the Lab
      • Methods for Microscopy
    • Research Projects >
      • hiPS Cells During Mitosis
      • Drug Perturbation Pilot
      • Differentiation Into Cardiomyocytes
      • Why Endogenous Tagging?
  • Data & Tools
    • Cell Catalog
    • 3D Cell Viewer
    • Cell Feature Explorer
    • Integrated Mitotic Stem Cell Z-stack Viewer
    • Deep Cell Zoom
    • Segmenter
    • Data Downloading
    • Software & Code
    • Simularium
    • Research Projects >
      • Extracting Information
  • Modeling & Analysis
    • Integrated Mitotic Stem Cell
    • Allen Integrated Cell
    • Label-free Determination
    • Data Notebook Exploration >
      • Cell Shape Analysis
      • Programmatic Data Access
    • Research Projects >
      • 3D Probabilistic Modeling
  • News & Publications
    • News Feed
    • Publications
    • Archived Content

Cell Structure Observations

Observations about microscopy videos for each of the 16 cell lines available in our Cell Catalog & 3D Cell Viewer.
Back to About our Cells

Histones visualized via Histone H2B type 1-J

8/1/2018

 
Movie. Z-stack of live hiPS cell colony expressing mEGFP-tagged Histone H2B type 1-J protein. Cells were imaged in 3D on a spinning-disk confocal microscope. Movie starts at the bottom of the cells and ends at the top. Scale bar, 5µm.
Movie. Time-lapse movie of live hiPS cell colony expressing mEGFP-tagged Histone H2B type 1-J protein. Cells were imaged in 3D on a spinning-disk confocal microscope every 3 min. A single mid-level z-section is shown. Movie plays at 1260x real time. Scale bar, 5 µm.
Movie. ​Z-stack of live hiPS cell colony expressing mEGFP-tagged Histone H2B type 1-J protein. Cell Mask Deep Red Plasma Membrane dye was applied to mark cell boundaries (magenta). NucBlue Live Ready Probe Reagent was used to compare mEGFP-tagged H2B localization (left panel) to a NucBlue Live (Hoechst) DNA stain (left panel). Cells were imaged in 3D on a spinning-disk confocal microscope. Movie starts at the bottom of the cells and ends at the top. Scale bar, 5µm.
Observations
  • Histone H2B type 1-J is a protein that participates in organizing DNA. It and other histone proteins form a core around which DNA wraps. These structures, called nucleosomes, are the fundamental repeating units within chromatin (DNA and histones). The packing of these structures impacts DNA accessibility, affecting functions like transcription, replication, and repair of DNA.
  • In hiPS cells imaged with spinning-disk light microscopy, mEGFP-tagged Histone H2B type 1-J appears within the nucleus as puncta of varying size and intensity, likely reflecting more densely packed regions of nucleosomes, and in a “haze”, where nucleosomes cannot be resolved.
  • The localization of mEGFP-tagged Histone H2B type 1-J is similar to the pattern of the DNA-binding dye Hoechst. Hoechst binds the minor grove of DNA (with preference for A-T rich regions), while mEGFP-tagged Histone H2B type 1-J localizes to the nucleosomal cores around which the DNA wraps.
  • Histone H2B type 1-J pattern of localization and intensity changes through the cell cycle. During interphase, Histone H2B type 1-J localizes throughout the nucleus, with some regions of greater intensity (reflecting a greater density of protein) near the nuclear periphery and the nucleolus. During cell division, Histone H2B type 1-J localizes to chromosomes.

    About

    Observations and descriptions from the microscope

    Archives

    February 2019
    August 2018
    April 2018
    October 2017
    April 2017
    March 2017

    Categories

    All
    3-channel
    3D Rotation
    Actin
    Actinomyosin
    Actin Structures
    Adhesions
    Cardiomyocyte
    Cell Cell Contacts
    Cell-cell Contacts
    Cell Contacts
    Cell Division
    Cell Membrane
    Centrin
    Centrioles
    Chromatin
    Colony
    Connexin
    Connexin-43
    Desmoplakin
    Desmosomes
    DNA
    Endoplasmic Reticulum
    Endosome
    ER
    Fibrillarin
    FUS
    Gap Junctions
    Golgi Apparatus
    H2B
    Histone
    K-Ras
    Lamin
    Lamin B1
    LAMP1
    Lysosomes
    MEGFP
    Membrane
    Microtubule
    Mitochondria
    Mitosis
    MLC-2a
    MLC 2v
    MTagRFP-T
    Muscle
    Myosin
    Nuclear
    Nuclear Envelope
    Nuclear Pores
    Nucleolus
    Nucleophosmin
    Nucleoporin
    Nucleus
    Nup153
    Paraspeckles
    Paxillin
    Peroxisome
    Plasma Membrane
    PMP34
    Rab-5A
    Ras
    RNA-binding Protein
    Sarcomere
    Sarcomere M-line
    Sarcomere Thick Filaments
    Sarcoplasmic Reticulum
    Sec61
    SERCA2
    Sialyltransferase 1
    SMC 1A
    ß-actin
    ST6GAL1
    Stress Granules
    Tight Junctions
    Time Lapse
    Time Series
    Titin
    Tom20
    Troponin
    Tubulin
    ZO1
    Z Stack

    RSS Feed

Home

Terms of Use

Citation Policy

Privacy Policy

FAQs

Help

Send us a message

Copyright © 2020 Allen Institute. All Rights Reserved.
cellscience.alleninstitute.org
  • About
    • What We Do
    • FAQs
    • Videos & Tutorials
    • Site Updates
    • < Forum >
    • < AllenInstitute.org >
  • Animated Cell
    • Educational Resources
    • Visual Guide to Human Cells
    • Visual Guide Tutorial
    • Research Projects >
      • Pathtrace rendering
  • Cells & Biology
    • Cell Catalog
    • About our Cells >
      • hiPS Cell Biology Overview
      • Cell Structure Observations
      • Cell Catalog QuickView
    • Genomics
    • Methods & SOPs >
      • Methods for Cells in the Lab
      • Cell Methods: Videos from the Lab
      • Methods for Microscopy
    • Research Projects >
      • hiPS Cells During Mitosis
      • Drug Perturbation Pilot
      • Differentiation Into Cardiomyocytes
      • Why Endogenous Tagging?
  • Data & Tools
    • Cell Catalog
    • 3D Cell Viewer
    • Cell Feature Explorer
    • Integrated Mitotic Stem Cell Z-stack Viewer
    • Deep Cell Zoom
    • Segmenter
    • Data Downloading
    • Software & Code
    • Simularium
    • Research Projects >
      • Extracting Information
  • Modeling & Analysis
    • Integrated Mitotic Stem Cell
    • Allen Integrated Cell
    • Label-free Determination
    • Data Notebook Exploration >
      • Cell Shape Analysis
      • Programmatic Data Access
    • Research Projects >
      • 3D Probabilistic Modeling
  • News & Publications
    • News Feed
    • Publications
    • Archived Content