Figures. Z-stack and timelapse movies of endosomes. A) Z-stack of a live hiPS cell colony expressing two copies (biallelic) of mEGFP-tagged Ras-related protein Rab-5A imaged in 3D on a spinning-disk confocal microscope. Movie starts at the bottom of the cells and ends at the top. Scale bar = 5µm. B) Timelapse movie of a live hiPS cell colony expressing two copies (biallelic) of mEGFP-tagged Ras-related protein Rab-5A. Cells were imaged on a spinning-disk confocal microscope. A single, mid-level plane was acquired every second. A zoom-in of the area boxed in the left panel is shown on the right. Movie sped up 10x real time. Scale bar = 5 µm.
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Observations and descriptions from the microscope