Figure 1. Z-stack and timelapse movie of nucleoli. The left panel shows a Z-stack of a live hiPS cell colony expressing mEGFP-tagged nucleophosmin. Cells were imaged in 3D on a spinning-disk confocal microscope. Right panel is the same image as the left but with contrast enhanced to visualize dimmer localization in mitotic cells. Movie starts at the bottom of the cells and ends at the top. Scale bar = 5µm.
Figure 2. Z-stack and timelapse movie of nucleoli. Timelapse movie of a live hiPS cell colony expressing mEGFP-tagged nucleophosmin. Cells were imaged on a spinning-disk confocal microscope every 3 min. Image is a maximum intensity projection through the volume of the cells. Frames were histogram matched to adjust for photobleaching. Movie plays at 900x real time. Scale bar = 5 µm.
Observations and descriptions from the microscope