Movie. Time-lapse movie of live hiPSC-derived cardiomyocytes expressing mEGFP-tagged titin protein. Twelve days after the onset of differentiation, cells were plated on PEI and laminin coated glass and imaged on a spinning disk confocal microscope 23 days later (35 days total after the onset of differentiation). A single plane of cells was imaged continuously with a 100 ms exposure time. Inset shows detail of titan in myofibrils. Scale bars, 10 µm. Movie plays in real time.
Observations and descriptions from the microscope